Antimicrobial effect of copper surfaces on bacteria isolated from poultry meat

Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens - Salmonella enterica and Listeria monocytogenes - and a poultry native microbiota bacterial species - Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results indicated that copper surfaces reduced the bacterial load quickly (<than 4 min) when the microorganisms were exposed to polished copper surfaces. Even when bacteria were inoculated on copper surfaces soiled with the organic matrix (washing water from poultry carcasses) and survival rates were significantly higher, an antimicrobial effect was still observed. Survival rates of two microorganisms simultaneously exposed to copper did not show significant differences. We found an antimicrobial effect over pathogenic and non-pathogenic microorganisms. Results suggest a potential role for copper surfaces in the control of microbiological hazards in the poultry industry.

Antimicrobial effect of copper surfaces on bacteria isolated from poultry meat

Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens Salmonella enterica and Listeria monocytogenes and a poultry native microbiota bacterial species Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results indicated that copper surfaces reduced the bacterial load quickly ( than 4 min) when the microorganisms were exposed to polished copper surfaces. Even when bacteria were inoculated on copper surfaces soiled with the organic matrix (washing water from poultry carcasses) and survival rates were significantly higher, an antimicrobial effect was still observed. Survival rates of two microorganisms simultaneously exposed to copper did not show significant differences. We found an antimicrobial effect over pathogenic and non-pathogenic microorganisms. Results suggest a potential role for copper surfaces in the control of microbiological hazards in the poultry industry.

Caracterización molecular y fisiológicas de levaduras de suelo trumao / Molecular and physiological characterization of yeasts of trumao soil

Las levaduras juegan un importante rol en la naturaleza siendo el mayor reservorio de ellas el suelo. Mediante el método de las diluciones seriadas y posterior siembra en agar Sabouraud se aislaron en cultivo puro 77 cepas de levaduras desde un mismo suelo trumao del sur de Chile, usado como pradera permanente (30 cepas), pradera en rotación (30 cepas) y como control bosque nativo (17 cepas), estas cepas se identificaron molecularmente por PCR-RFLP en conjunto con secuenciación del rDNA de ITS-5.8S, además se realizo una caracterización fisiológica (asimilación fuente de carbono, de nitrógeno y fermentación de azucares) a cada cepa. Mediante las técnicas moleculares las 77 cepas se reunieron en 10 grupos, de estos solamente tres grupos se pudieron identificar a nivel de especie y uno hasta género: Devariomyces hansenii. Pichia fermentan. Kazachstania exigua., Candida sp.

Yeasts plays an important role in nature, It is the largest reservoir of soil them. By the method of serial dilutions and subsequent planting in Sabouraud agar were isolated in pure culture 77 strains of yeast from the same volcanic ash soil of southern Chile, used as permanent pasture (30 strains), rotation pasture (30 strains) and native forest as a control (17 strains), these strains were identified molecularly by PCR-RFLP in conjunction with rDNA sequencing ITS-5.8S, physiological characterization addition was performed to each strain (carbon and nitrogen source assimilation and fermentation of sugars). Using molecular techniques met the 77 strains in 10 groups; only three groups could be identified to species level and one to gender: Devariomyces hansenii; Pichia fermented; Kazachstania exigua; Candida sp.

Identification of Lactobacillus spp. in colostrum from Chilean mothers

The biodiversity of Lactobacillus spp. in colostrum samples from 116 Chilean mothers was analyzed by PCR and 16S rDNA sequencing. Lactobacilli were isolated in 55.3% of the samples, with concentrations of 3.33 ± 0.55 (log CFU/ml). The predominant species were L. plantarum (64%), L. fermentum (16%) and L. pentosus (9%). 28% of the isolated strains were resistant to gastric pH and bile salts, suggesting that they could be used as probiotics.

Se analizó la biodiversidad de especies de Lactobacillus en muestras de calostro de 116 madres chilenas mediante PCR y secuenciación del rDNA 16S. Se aislaron lactobacilos en 55,3% de las muestras, con concentraciones de 3,33 ± 0,55 (log UFC / ml). Las especies predominantes fueron L. plantarum (64%), L. fermentum (16%) y L. pentosus (9%). 28% de las cepas aisladas fueron resistentes a pH gástrico y a las sales biliares, lo que sugiere que podrían ser utilizados como probióticos.